Application
Western blot analysis of Thy-1 expression in non-transfected 293T: (A), human Thy-1 transfected 293T: (B) and mouse PBL (C) whole cell lysates.Application
Western blot analysis of Thy-1 expression in non-transfected: (A) and human Thy-1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Thy-1 expression in IMR-32 (A), CCRF-CEM (B) and MOLT-4 (C) whole cell lysates and rat brain tissue extract (D).Application
Western blot analysis of Thy-1 expression in SUP-T1 whole cell lysate.| Product Name | Thy-1 Antibody (H-110) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 20-130 representing mature Thy-1 of human origin |
| Modification Notes | Over 100 cell surface markers have been identified through the use of monoclonal antibodies. Many of these markers have proven useful in identifying specific subpopulations of cells within mixed colonies. Accordingly, these molecules have been assigned a “cluster of differentiation” (CD) designation. One such marker, designated Thy-1 (also referred to as CDw90), is a phosphatidyl-anchored cell surface glycoprotein which, when coexpressed with CD34 on cells from normal human bone marrow, identifies a subpopulation that includes putative hematopoietic, pleuripotent stem cells. Thy-1+ cells from bone marrow have been implicated in syngeneic graft versus host disease and may serve to regulate autoreactivity after bone marrow transplant. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Thy-1 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 20-130 representing mature Thy-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 20-130 representing mature Thy-1 of human origin |
| Entrez Gene | 7070 |
|---|
Application
Western blot analysis of Thy-1 expression in non-transfected 293T: (A), human Thy-1 transfected 293T: (B) and mouse PBL (C) whole cell lysates.
Application
Western blot analysis of Thy-1 expression in non-transfected: (A) and human Thy-1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Thy-1 expression in IMR-32 (A), CCRF-CEM (B) and MOLT-4 (C) whole cell lysates and rat brain tissue extract (D).
Application
Western blot analysis of Thy-1 expression in SUP-T1 whole cell lysate.| Application Notes | recommended for detection of Thy-1 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
