Application
Western blot analysis of SVCT2 expression in non-transfected: (A) and mouse SVCT2 transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.| Product Name | SVCT2 Antibody (H-70) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 581-650 mapping at the C-terminus of SVCT2 of human origin |
| Modification Notes | The sodium-dependent vitamin C transporters SVCT1 and SCVT2 are membrane transporters for L-ascorbic acid (Vitamin C) (1-4). Both SVCT proteins mediate high affinity Na+-dependent L-ascorbic acid transport and are necessary for the uptake of vitamin C in many tissues (1-4). SVCT1 is a 604 amino acid protein that is expressed mainly in epithelial tissues, including intestine, kidney, and liver (2,3). SVCT2 is a 592 amino acid protein that shares 65% homology to SVCT1 and has been detected in various metabolically active cells as well as in specialized tissues such as eye and brain (1,4). A non-functional splice variant of SVCT1 has been identified in normal human intestine (5). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of SVCT2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 581-650 mapping at the C-terminus of SVCT2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 581-650 mapping at the C-terminus of SVCT2 of human origin |
| Entrez Gene | 9962 |
|---|
Application
Western blot analysis of SVCT2 expression in non-transfected: (A) and mouse SVCT2 transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.| Application Notes | recommended for detection of SVCT2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
