Application
Western blot analysis of SOCS-3 expression in human PBL whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human ovary tissue showing cytoplasmic staining of ovarian stroma cells.Application
Immunofluorescence staining of methanol-fixed HeLa + IFN-γ cells showing cytoplasmic localization.| Product Name | SOCS-3 Antibody (H-103) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 87-189 of SOCS-3 of human origin |
| Modification Notes | The SOCS (suppressor of cytokine signaling) gene family consists of a group of proteins that negatively regulate cytokine signal transduction (1). The SOCS family proteins contain a central SH2 domain and a carboxy-terminal region termed the “SOCS box.” The SOCS-1 (also called SSI-1 and JAB), SOCS-2 (also called SSI-2 and CIS2) and SOCS-3 (also called SSI-3 and CIS3) genes are known to be upregulated by IL-6 and other cytokines (2). SOCS-4, SOCS-5, SOCS-6 and SOCS-7 were identified by their sequence homology with the SOCS box (3). CIS (for cytokine-inducible SH2-containing protein) is also a member of the SOCS family (4,5). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of SOCS-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 87-189 of SOCS-3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 87-189 of SOCS-3 of human origin |
| Entrez Gene | 9021 |
|---|
Application
Western blot analysis of SOCS-3 expression in human PBL whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human ovary tissue showing cytoplasmic staining of ovarian stroma cells.
Application
Immunofluorescence staining of methanol-fixed HeLa + IFN-γ cells showing cytoplasmic localization.| Application Notes | recommended for detection of SOCS-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
