Application
Western blot analysis of Annexin VI expression in JAR whole cell lysate.Application
Western blot analysis of Annexin VI expression in MES-SA/Dx5 (A), JAR (B) and JEG-3 (C) whole cell lysates.| Product Name | Annexin VI Antibody (H-114) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 497-610 mapping near the C-terminus of Annexin VI of human origin |
| Modification Notes | The annexin family of calcium-binding proteins is composed of at least ten mammalian genes. It is characterized by a conserved core domain, which binds to phospholipids in a Ca++-dependent manner, and a unique amino terminal region, which may confer binding specificity. The annexin family has been implicated as regulators of such diverse processes as ion-flux, endocytosis and exocytosis, and cellular adhesion. When overexpressed in A-431 cells, Annexin VI causes a partial reversal of the transformed phenotype. It has been hypothesized that growth-dependent posttranslational modifications of Annexins are required for proper sub-cellular localization. Annexin VII, also referred to as synexin, is located at the plasma membrane in nor-mal muscle tissue. However, in muscle samples from patients suffering from Duchenne’s muscular dystrophy, Annexin VII, along with Annexins IV and VI, is released into the cytoplasm and later, as the disease progresses, into the extracellular space. Two forms of Annexin XI, designated A and B, have been identified. Transfection of COS-7 cells with Annexin XI-A, but not Annexin XI-B, causes formation of Annexin XI-associated vesicles. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Annexin VI of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 497-610 mapping near the C-terminus of Annexin VI of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 497-610 mapping near the C-terminus of Annexin VI of human origin |
| Entrez Gene | 309 |
|---|
Application
Western blot analysis of Annexin VI expression in JAR whole cell lysate.
Application
Western blot analysis of Annexin VI expression in MES-SA/Dx5 (A), JAR (B) and JEG-3 (C) whole cell lysates.| Application Notes | recommended for detection of Annexin VI of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
