Application
Western blot analysis of Annexin I expression in non-transfected: (A) and mouse Annexin I transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Annexin I expression in non-transfected: (A) and mouse Annexin I transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Annexin I expression in K-562 whole cell lysate.Application
Western blot analysis of Annexin I expression in A-431 (A), K-562 (B) and Caki-1 (C) whole cell lysates.Application
Western blot analysis of human recombinant Annexin I fusion protein.| Product Name | Annexin I Antibody (H-65) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 235-299 of Annexin I of human origin |
| Modification Notes | The annexin family of calcium-binding proteins is composed of at least ten mammalian genes and is characterized by a conserved core domain which binds phospholipids in a Ca2+-dependent manner and a unique amino terminal region which may confer binding specificity. The interaction between these proteins and biological membranes have led to the hypothesis that they are involved in cellular trafficking processes such as endocytosis, exocytosis and cellular adhesion. Annexin I, alternatively referred to as lipocortin, has been implicated as a mediator of the anti-inflammatory response produced by glucocorticoids and as an inhibitor of cPLA2, a potent mediator of inflammation. Annexin II, also called p36, has been shown to exist as a monomer or a heterotetramer, complexed with the S-100-related protein p11. This complex is termed calpactin I. In the tetrameric form, Annexin II is an efficient substrate of the PKC family and Src pp60. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Annexin I of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 235-299 of Annexin I of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 235-299 of Annexin I of human origin |
| Entrez Gene | 301 |
|---|
Application
Western blot analysis of Annexin I expression in non-transfected: (A) and mouse Annexin I transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Annexin I expression in non-transfected: (A) and mouse Annexin I transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Annexin I expression in K-562 whole cell lysate.
Application
Western blot analysis of Annexin I expression in A-431 (A), K-562 (B) and Caki-1 (C) whole cell lysates.
Application
Western blot analysis of human recombinant Annexin I fusion protein.| Application Notes | recommended for detection of Annexin I of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
