Application
Western blot analysis of COPG expression in non-transfected 293T: (A), mouse COPG transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Western blot analysis of COPG expression in non-transfected 293T: (A), mouse COPG transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Western blot analysis of COPG expression in non-transfected 293T: (A), human COPG transfected 293T: (B) and K-562 (C) whole cell lysates.| Product Name | COPG Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 575-874 mapping at the C-terminus of COPG of human origin |
| Modification Notes | Membrane and vesicular trafficking in the early secretory pathway are mediated by non-clathrin COP (coat protein) I-coated vesicles. COPI-coated vesicles mediate retrograde transport from the Golgi back to the ER and intra-Golgi transport. The cytosolic precursor of the COPI coat, the heptameric coatomer complex, is composed of two subcomplexes. The first consists of the COPB, COPG, COPD and COPZ subunits (also known as b-, g-, d- and z-COP, respectively), which are distantly homologous to AP Clathrin adaptor subunits. The second consists of the COPA, b'-COP and COPE subunits (also known as a-COP, COPP and e-COP, r espectively). The COPG (g-COP) subunit of the coatomer is believed to mediate the binding to the cytoplasmic dilysine motifs of membrane proteins. COPG has the highest level of expression in mouse testis and is expressed in a parent-of-origin-specific manner in mammals. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of COPG (also designated γ-COP) and γ2-COP of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 575-874 mapping at the C-terminus of COPG of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 575-874 mapping at the C-terminus of COPG of human origin |
| Entrez Gene | 22820 |
|---|
Application
Western blot analysis of COPG expression in non-transfected 293T: (A), mouse COPG transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Western blot analysis of COPG expression in non-transfected 293T: (A), mouse COPG transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Western blot analysis of COPG expression in non-transfected 293T: (A), human COPG transfected 293T: (B) and K-562 (C) whole cell lysates.| Application Notes | recommended for detection of COPG (also designated γ-COP) and γ2-COP of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
