Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing cytoplasmic staining of neuronal cells.Application
Western blot analysis of IscU1/2 expression in non-transfected: (A) and human IscU1/2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of IscU1/2 expression in non-transfected: (A) and mouse IscU1/2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of IscU1/2 expression in non-transfected: (A) and human IscU1/2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of IscU1 and IscU2 expression in HeLa (A), SW480 (B) and HCT 116 (C) whole cell lysates.| Product Name | IscU1/2 Antibody (FL-142) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-142 representing full length IscU1 of human origin |
| Modification Notes | Iron-sulfur (Fe-S) clusters are cofactors that are essential for a wide variety of processes, including facilitation of electron transfer processes in oxidative phosphorylation, catalysis of enzymatic reactions in aconitase and dehydratases and maintenance of structural integrity in the DNA repair enzyme endonuclease III. In bacteria and eukaryotes, several new genes are implicated in the biogenesis of Fe-S cluster-containing proteins. IscU1 and IscU2, homologs to bacterial IscU and NifU, are iron cluster-assembly proteins. Deletion of either IscU1 or IscU2 results in increased accumulation of iron within the mitochondria, loss of activity of the [4Fe-4S] aconitase enzyme, and suppression of oxidative damage in cells lacking cytosolic copper/zinc superoxide dismutase. IscU1 and IscU2 are regulated by the iron status of the cell and localize primarily in the mitochondria. In human cells, alternative splicing of IscU pre-mRNA results in synthesis of these two proteins, which differ at the N-terminus and localize either to the cytosol (IscU1) or the mitochondria (IscU2). IscU proteins interact with IscS, a cysteine desulfurase, to sequester inorganic sulfur for Fe-S cluster assembly. IscU-IscS protein complex localizes in both mitochondria and cytosol, implying that Fe-S cluster assembly takes place in multiple subcellular compartments in mammalian cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of IscU1/2 of human origin and IscU of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-142 representing full length IscU1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-142 representing full length IscU1 of human origin |
| Entrez Gene | 23479 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing cytoplasmic staining of neuronal cells.
Application
Western blot analysis of IscU1/2 expression in non-transfected: (A) and human IscU1/2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of IscU1/2 expression in non-transfected: (A) and mouse IscU1/2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of IscU1/2 expression in non-transfected: (A) and human IscU1/2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of IscU1 and IscU2 expression in HeLa (A), SW480 (B) and HCT 116 (C) whole cell lysates.| Application Notes | recommended for detection of IscU1/2 of human origin and IscU of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
