Application
Western blot analysis of ASIC1 expression in mouse brain (A), mouse cerebellum (B) and mouse pituitary (C) tissue extracts.Application
Western blot analysis of ASIC1 expression in MCF7 whole cell lysate.Application
Western blot analysis of ASIC1 expression in mouse brain (A) and rat brain (B) tissue extracts.Application
Western blot analysis of ASIC1 expression in U-87 MG whole cell lysate.| Product Name | ASIC1 Antibody (H-70) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 505-574 mapping at the C-terminus of ASIC1 of human origin |
| Modification Notes | Degenerin/epithelial sodium channel (DEG/ENaC) superfamily members are amiloride-sensitive sodium channels that contain intracellular N- and C-termini, two hydrophobic transmembrane regions and a cysteine-containing extracellular loop. Acid sensing ion channel ASIC1, also designated ACCN2, BNAC2 and ASIC1a, is present in brain as a 4.3-kb transcript with localization to rat dorsal root ganglia. In situ hybridization of rat brain suggests that ASIC1 is most abundant in the main olfactory bulb, cerebral cortex, hippocampal formation, habenula, basolateral amygdaloid nuclei and cerebellum. ASIC1 and H+-gated currents may contribute to the development of fear and anxiety. ASIC2, also designated amiloride-sensitive cation channel 1, neuronal (ACCN1), mammalian degenerin, BNAC1 (MDEG) and brain Na+ channel 1, mediates the normal detection of light touch. ASIC2 mRNA is abundant in brain, specifically in neurons. ASIC2 is expressed as 2.7- and 3.7-kb transcripts in brain and spinal cord tissues. ASIC3, also designated SLNAC1 and TNaC1, mediates detection of lasting pH changes and is involved in modulating moderate- to high-intensity pain sensation. ASIC4, also designated ACCN4 and BNAC4, is abundant in pituitary gland and is also present in the inner ear. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of ASIC1 of human origin and ASIC1 isoforms α and β of mouse and rat origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 505-574 mapping at the C-terminus of ASIC1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 505-574 mapping at the C-terminus of ASIC1 of human origin |
| Entrez Gene | 41 |
|---|
Application
Western blot analysis of ASIC1 expression in mouse brain (A), mouse cerebellum (B) and mouse pituitary (C) tissue extracts.
Application
Western blot analysis of ASIC1 expression in MCF7 whole cell lysate.
Application
Western blot analysis of ASIC1 expression in mouse brain (A) and rat brain (B) tissue extracts.
Application
Western blot analysis of ASIC1 expression in U-87 MG whole cell lysate.| Application Notes | recommended for detection of ASIC1 of human origin and ASIC1 isoforms α and β of mouse and rat origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
