Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of FRS2 expression in NIH/3T3 whole cell lysate.| Product Name | FRS2 Antibody (H-91) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 258-348 of FRS2 of human origin |
| Modification Notes | FRS2 (also designated SNT or p90) is a lipid-anchored docking protein that becomes tyrosine phosphorylated in response to FGF or NGF stimulation and subsequently binds to GRB2/Sos complexes (1-4). The GRB2 adapter protein links receptor tyrosine kinases to the Ras/MAPK signaling pathway but does not interact directly with FGF receptors (5,6). FRS2 thus provides a link between activation of FGF and NGF receptors and the Ras/MAPK pathway. FRS2 contains four Grb2 binding sites, a myristylation sequence and a PTP domain (1). Myristylation of FRS2 is essential for membrane localization, tyrosine phosphorylation, GRB2/Sos recruitment and MAPK activation (1). The function of FRS2 in FGF receptor signaling is analogous to that of IRS1 in response to insulin receptor stimulation (7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of FRS2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 258-348 of FRS2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 258-348 of FRS2 of human origin |
| Entrez Gene | 10818 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of FRS2 expression in NIH/3T3 whole cell lysate.| Application Notes | recommended for detection of FRS2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
