Application
Western blot analysis of IL-7R expression in K-562 (A), WEHI-231 (B), Daudi (C), BJAB (D), AML-193 (E) and Jurkat (F) whole cell lysates.| Product Name | IL-7R Antibody (H-215) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-215 mapping at the C-terminus of IL-7R of human origin |
| Modification Notes | Interleukin 7 (IL-7) was originally described as a factor capable of inducing in vitro proliferation of pre-B cells from marrow cultures. The IL-7 gene encodes a protein 177 amino acids in length. IL-7 exerts its biological function through the IL-7 receptor which is expressed on pre-B cells, thymocytes and bone marrow-derived macrophages. The IL-7 receptor is composed of an IL-7 receptor-specific chain and the IL-2 receptor g chain common to the IL-2, IL-4, IL-7, IL-9 and IL-15 receptors. IL-7 stimulation leads to the activation of Janus tyrosine kinase family members JAK1 and JAK3. Other studies have shown that in T cells, the IL-7 receptor-specific chain associates with the Src kinases family Lck and Fyn. IL-7 induces phosphorylation of insulin receptor substrate-1 (IRS-1) and insulin receptor substrate-2 (IRS-2), originally called 4PS. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of IL-7R of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-215 mapping at the C-terminus of IL-7R of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-215 mapping at the C-terminus of IL-7R of human origin |
| Entrez Gene | 3575 |
|---|
Application
Western blot analysis of IL-7R expression in K-562 (A), WEHI-231 (B), Daudi (C), BJAB (D), AML-193 (E) and Jurkat (F) whole cell lysates.| Application Notes | recommended for detection of IL-7R of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
