Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of IL-4Rα expression in Ramos whole cell lysate.| Product Name | IL-4Rα Antibody (C-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within a C-terminal cytoplasmic domain of IL-4Rα of human origin |
| Modification Notes | The IL-2 receptor is a multicomponent complex consisting of three subunits, å, ∫ and ©, each of which is required for high affinity binding of IL-2 (1–3). The a chain functions primarily in binding IL-2, whereas the ∫ and © chains contribute to IL-2 binding and are essential to IL-2-induced activation of signaling pathways leading to T cell growth (4). Both IL-4R and IL-7R were initially described as single chain, high-affinity ligand-binding cytokine receptors. However, it is now well established that the IL-2R© chain functions as a second subunit of the high affinity IL-4R and IL-7R receptors (4,6). Consequently, the originally described subunits of these latter receptors are now referred to as IL-4Rå (5) and IL-7Rå (6), respectively, while the common subunit is referred to as ©c (4). Although the common © chain enhances ligand binding in these three cytokine receptors, it has no capacity to bind these ligands on its own (1,7). There is evidence that the ©c chain is also a subunit of IL-13R (7,8). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of IL-4Rα chain of human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within a C-terminal cytoplasmic domain of IL-4R&alpha of human origin |
|---|---|
| Tissue Specificity | epitope mapping within a C-terminal cytoplasmic domain of IL-4Rα of human origin |
| Entrez Gene | 3566 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of IL-4Rα expression in Ramos whole cell lysate.| Application Notes | recommended for detection of IL-4Rα chain of human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
