Application
Western blot analysis of human recombinant TNFα.| Product Name | TNFα Antibody (H-156) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 77-233 representing mature tumor necrosis factor alpha (TNFα) of human origin |
| Modification Notes | Tumor necrosis factor ∫ (TNF∫), also known as lymphotoxin, is a pleiotropic cytokine. TNFå, also known as cachectin, is a smaller cytokine that binds to the same receptors, producing a vast array of effects similar to those of TNF∫. TNF∫ and TNFå share 30% amino acid homology and have similar biological activities. TNF∫ is produced by activated lymphocytes, including CD4+ T helper cell type 1 lymphocytes, CD8+ lymphocytes and certain B lymphoblastoid cell lines. TNFå is produced by several different cell types, which include lymphocytes, neutrophils and macrophages. TNFå and TNF∫ can modulate many immune and inflammatory functions, while having the ability to inhibit tumor growth. Target tumor cells must express TNF receptors 1 and 2 to be killed, with the p55 receptor mediating the cytotoxic response. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| WB:1:100~1:200 IP:1:20~1:100 IF:1:20~1:100 |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | equine, canine and porcine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 77-233 representing mature tumor necrosis factor alpha (TNF&alpha ) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 77-233 representing mature tumor necrosis factor alpha (TNFα) of human origin |
| Entrez Gene | 7124 |
|---|
Application
Western blot analysis of human recombinant TNFα.| Application Notes | WB:1:100~1:200 IP:1:20~1:100 IF:1:20~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
