Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of EphA4 expression in non-transfected: (A) and mouse EphA4 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of EphA4 expression in mouse brain tissue extract.Application
Intracellular FCM analysis of fixed and permeabilized NIH/3T3 cells. Black line histogram represents the isotype control, normal rabbit IgG: .Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tissue showing membrane and cytoplasmic staining.Application
Western blot analysis of EphA4 expression in rat brain extract.| Product Name | EphA4 Antibody (S-20) PE |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping near the C-terminus of EphA4 of human origin |
| Modification Notes | The Eph subfamily represents the largest group of receptor protein tyrosine kinases identified to date (1–3). While the biological activities of these receptors have yet to be determined, there is increasing evidence that they are involved in central nervous system function and in development (1–3). The Eph subfamily receptors of human origin (and their murine/avian homologs) include EphA1 (Eph), EphA2 (Eck), EphA3 (Hek4), EphA4 (Hek8), EphA5 (Hek7), EphA6 (Hek12), EphA7 (Hek11/MDK1), EphA8 (Hek3), EphB1 (Hek6), EphB2 (Hek5), EphB3 (Cek10, Hek2), EphB4 (Htk), EphB5 (Hek9) and EphB6 (Mep). Ligands for Eph receptors include ephrin-A4 (LERK-4) which binds EphA3 and EphB1. In addition, ephrin-A2 (ELF-1) has been described as the ligand for EphA4, ephrin-A3 (Ehk1-L) as the ligand for EphA5 and ephrin-B2 (Htk-L) as the ligand for EphB4 (Htk) (4–7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCFCELISA |
| recommended for detection of EphA4 of mouse, rat, human and avian origin by WB, IP, IF, IHC(P), FCM and ELISA also reactive with additional species, including equine, canine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the C-terminus of EphA4 of human origin |
|---|---|
| Tissue Specificity | epitope mapping near the C-terminus of EphA4 of human origin |
| Entrez Gene | 2043 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of EphA4 expression in non-transfected: (A) and mouse EphA4 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of EphA4 expression in mouse brain tissue extract.
Application
Intracellular FCM analysis of fixed and permeabilized NIH/3T3 cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tissue showing membrane and cytoplasmic staining.
Application
Western blot analysis of EphA4 expression in rat brain extract.| Application Notes | recommended for detection of EphA4 of mouse, rat, human and avian origin by WB, IP, IF, IHC(P), FCM and ELISA also reactive with additional species, including equine, canine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
