Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing nuclear and cytoplasmic staining of cells in seminiferous ducts.Application
Western blot analysis of Cdc2 p34 expression in non-transfected 293T: (A), mouse Cdc2 p34 transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Western blot analysis of Cdc2 p34 expression in non-transfected 293: (A), human Cdc2 p34 transfected 293: (B) and HeLa (C) whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing nuclear and cytoplasmic staining.Application
Western blot analysis of Cdc2 p34 expression in A-431 (A) and HeLa (B) nuclear extracts.Application
Blots were probed with p-Cdc2 p34 (Thr 14/Tyr 15)-R: preincubated with cognate phosphorylated (A) or cognate unphosphorylated (B) peptide and Cdc2 p34 (H-297): (C).| Product Name | Cdc2 p34 Antibody (H-297) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-297 representing full length Cdc2 p34 of human origin |
| Modification Notes | In vertebrates, as in yeast, multiple cyclins have been identified, including a total of eight such regulatory proteins in mammals. In contrast to the situation in yeast, the Cdc2 p34 kinase is not the only catalytic subunit identified in vertebrates that can interact with cyclins. While Cdc2 p34 is essential for the G2 to M transition in vertebrate cells, a second Cdc2-related kinase has also been implicated in cell cycle control. This protein, designated cyclin-dependent kinase 2 (Cdk2) p33, also binds to cyclins and its kinase activity is temporally regulated during the cell cycle. Several additional Cdc2 p34-related cyclin dependent kinases have been identified. These include Cdk3-Cdk8, PCTAIRE-1-3 and KKIALRE. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Cdc2 p34 of mouse, rat, human and Xenopus origin by WB, IP, IF, IHC(P) and ELISA minimal cross-reactivity with other cyclin dependent kinases also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-297 representing full length Cdc2 p34 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-297 representing full length Cdc2 p34 of human origin |
| Entrez Gene | 983 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing nuclear and cytoplasmic staining of cells in seminiferous ducts.
Application
Western blot analysis of Cdc2 p34 expression in non-transfected 293T: (A), mouse Cdc2 p34 transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Western blot analysis of Cdc2 p34 expression in non-transfected 293: (A), human Cdc2 p34 transfected 293: (B) and HeLa (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing nuclear and cytoplasmic staining.
Application
Western blot analysis of Cdc2 p34 expression in A-431 (A) and HeLa (B) nuclear extracts.
Application
Blots were probed with p-Cdc2 p34 (Thr 14/Tyr 15)-R: preincubated with cognate phosphorylated (A) or cognate unphosphorylated (B) peptide and Cdc2 p34 (H-297): (C).| Application Notes | recommended for detection of Cdc2 p34 of mouse, rat, human and Xenopus origin by WB, IP, IF, IHC(P) and ELISA minimal cross-reactivity with other cyclin dependent kinases also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
