Application
Western blot analysis of Integrin αX expression in non-transfected: (A) and human Integrin αX transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Integrin αX expression in PMA-treated K-562 whole cell lysate.| Product Name | Integrin αX Antibody (H-68) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 613-680 mapping within an extracellular domain of Integrin αX of human origin |
| Modification Notes | Integrin åX (CD11C, leukocyte surface antigen p150,95, CR4, Axb2) is a type 1 transmembrane protein that traditionally combines with ∫2 chain to form a leukocyte-specific integrin known as inactivated-C3b (iC3b) receptor 4 (CR4). Integrin åX/∫2 shares similar properties of the åM/∫2 integrin in mediating adherence of neutrophils and monocytes to stimulated endothelial cells, and in phagocytosis of complement coated particles. Abnormal expression of Integrin åX is characteristic of hairy cell leukemia (HCL) and is dependent upon activation of proto-oncogenes Ras and JunD. Proteins and DNA elements that influence transcription of Integrin åX include Sp1 and Sp1-like factors, AP-1 family, C/EBP, Oct-2 and PU.1. Integrin åX is present on monocyte derivative dendritic cells (DCs), macrophages and NK cells. Upon activation, DCs present in skin (Langerhans cells), lining of nose, lung, stomach, intestine and blood can migrate to lymphoid tissues and interact with T and B cells to initiate and shape the immune response. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Integrin αX of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 613-680 mapping within an extracellular domain of Integrin &alpha X of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 613-680 mapping within an extracellular domain of Integrin αX of human origin |
| Entrez Gene | 3687 |
|---|
Application
Western blot analysis of Integrin αX expression in non-transfected: (A) and human Integrin αX transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Integrin αX expression in PMA-treated K-562 whole cell lysate.| Application Notes | recommended for detection of Integrin αX of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
