Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing cytoplasmic and nuclear staining of squamous epithelial cells.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing cytoplasmic and nuclear staining of surface epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of Calgranulin B expression in untreated (A) and DMSO-treated HL-60 (B) whole cell lysates.| Product Name | Calgranulin B Antibody (H-90) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 25-114 mapping at the C-terminus of Calgranulin B of human origin |
| Modification Notes | The family of EF-hand type Ca2+-binding proteins includes Calbindin (previously designated vitamin D-dependent Ca2+-binding protein), S-100α and β, Calgranulin A (also designated MRP8), Calgranulin B (also designated MRP14) and Calgranulin C (S-100 like protein), and the parvalbumin family members, including parvalbumin α and parvalbumin β (also designated oncomodulin). Calbindin, S-100 proteins and parvalbumin proteins are each expressed in neural tissues. In addition, S-100α and β are present in a variety of other tissues, and Calbindin is present in intestine and kidney. Parvalbumin α is also found in fast-contracting/relaxing skeletal muscle fibers and parvalbumin β is found in many tumor tissues as well as in the organ of Corti. Calbindin, S-100 proteins and parvalbulmins have all been detected in leydig cells and testis. These proteins are thought to play a role in hormone production and spermatogenesis. Calgranulin is expressed in macrophages and epithelial cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Calgranulin B of human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 25-114 mapping at the C-terminus of Calgranulin B of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 25-114 mapping at the C-terminus of Calgranulin B of human origin |
| Entrez Gene | 6280 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing cytoplasmic and nuclear staining of squamous epithelial cells.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing cytoplasmic and nuclear staining of surface epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of Calgranulin B expression in untreated (A) and DMSO-treated HL-60 (B) whole cell lysates.| Application Notes | recommended for detection of Calgranulin B of human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
