Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing nuclear and cytoplasmic staining of glandular cells.Application
Immunofluorescence staining of normal mouse kidney frozen section showing cytoplasmic staining.| Product Name | BRCA2 Antibody (H-299) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | In 1990, a breast cancer susceptibility gene designated BRCA1 was localized to chromosome 17q. Mutations within this gene are believed to account for approximately 45% of families with high incidence of breast cancer and at least 80% of families with increased incidence of both early-onset breast cancer and ovarian cancer. A second breast cancer susceptibility gene, BRCA2 (breast cancer 2, early onset), located on chromosome 13q13.1, also confers a high incidence of breast cancer but, unlike BRCA1, does not confer a substantially elevated risk of ovarian cancer. Both BRCA1 and BRCA2 play a role in the maintainance of genome stability, particularly in the homologous recombination pathway for double-strand DNA repair. BRCA2 is regarded as a tumor suppressor gene; tumors with BRCA2 mutations exhibit loss of heterozygosity (LOH) of the wildtype allele. The protein encoded by the BRCA2 gene contains multiple copies of a 70 amino acid motif called the BRC motif. These motifs effect binding to the Rad51 recombinase, which operates in DNA repair. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCIPWB |
| WB:1:100~1:200 IHC:1:20~1:50 IF:1:20~1:100 ELISA:1:1000 IP:1:20~1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-299 mapping at the N-terminus of BRCA2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-299 mapping at the N-terminus of BRCA2 of human origin |
| Entrez Gene | 675 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing nuclear and cytoplasmic staining of glandular cells.
Application
Immunofluorescence staining of normal mouse kidney frozen section showing cytoplasmic staining.| Application Notes | WB:1:100~1:200 IHC:1:20~1:50 IF:1:20~1:100 ELISA:1:1000 IP:1:20~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
