Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human small intestine tissue showing cytoplasmic and nuclear staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of cGKIα/β expression in NIH/3T3 (A) and CTLL-2 (B) whole cell lysates.| Product Name | cGKIα/β Antibody (H-100) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 191-290 mapping to an internal region of cGKIα of human origin |
| Modification Notes | cGKI (cGMP-dependent protein kinase type I), also known as PRKG1, lowers the intracellular level of calcium and is important for the relaxation of vascular smooth muscle. cGKI exists as two alternatively spliced isoforms, designated å and ∫, which differ only in their N-terminal sequence and function to catalyze the phosphorylation of target proteins. The cGKIå/∫ precursor contains one protein kinase domain, one AGC-kinase C-terminal domain and two cyclic nucleotide-binding domains. cGKII (cGMP-dependent protein kinase type II), a protein that is related to cGKI, is a major receptor of intracellular cGMP that mediates a plethora of physiological responses. cGKII contains a conserved leucine zipper motif at the amino terminus and is expressed in small intestine, colon, prostate and human brain tissue. cGKII has been shown to regulate the ion transport system in the intestine. Myristoylation of the penultimate glycine in cGKII appears to be essential for directing cGKII to the membrane, since cGKII is devoid of any hydrophobic transmembrane domains. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of cGKIα and β of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 191-290 mapping to an internal region of cGKI&alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 191-290 mapping to an internal region of cGKIα of human origin |
| Entrez Gene | 5592 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human small intestine tissue showing cytoplasmic and nuclear staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of cGKIα/β expression in NIH/3T3 (A) and CTLL-2 (B) whole cell lysates.| Application Notes | recommended for detection of cGKIα and β of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
