Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bronchus tissue showing cytoplasmic and nuclear staining of respiratory epithelial cells.Application
Western blot analysis of PERK expression in NIH/3T3 whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human thyroid gland tissue showing cytoplasmic and nuclear staining of glandular cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.| Product Name | PERK Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 21-320 mapping near the N-terminus of PERK of human origin |
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | An interferon-inducible, RNA-dependent protein serine/threonine kinase (PKR) has been described. PKR in earlier literature is variously known as DAI, dsJ, PI kinase, p65, p67 or TIK for the mouse kinase; and p68 or p69 for the human kinase. The PKR kinase substrate is the å subunit of protein synthesis initiation factor eIF-2. Phosphorylation of eIF-2å on serine-51 results in inhibition of translation. The serine/threonine kinase catalytic domains map to the carboxy terminal half of the protein while the RNA-binding domains are located in the amino terminal region. PERK is a type I transmembrane protein located in the endoplasmic reticulum (ER) that contains a kinase domain similar to the kinase domain of PKR. PERK is activated in response to ER stress and phosphorylates eIF-2å, thus inhibiting the translation of mRNA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCIPWB |
| WB:1:100~1:500 IP:1:20~1:100 IF:1:20~1:100 IHC:1:10~1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 21-320 mapping near the N-terminus of PERK of human origin |
|---|---|
| Molecular Weight(MW) | 125 KD |
| Tissue Specificity | epitope corresponding to amino acids 21-320 mapping near the N-terminus of PERK of human origin |
| Entrez Gene | 9451 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bronchus tissue showing cytoplasmic and nuclear staining of respiratory epithelial cells.
Application
Western blot analysis of PERK expression in NIH/3T3 whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human thyroid gland tissue showing cytoplasmic and nuclear staining of glandular cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.| Application Notes | WB:1:100~1:500 IP:1:20~1:100 IF:1:20~1:100 IHC:1:10~1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
