Application
Western blot analysis of Ctk expression in BC3H1 (A) and EOC 20 (B) whole cell lysates.| Product Name | Ctk Antibody (C-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | All members of the Src gene family of tyrosine kinases are characterized by a carboxy terminal domain tyrosine, Y527 in the case of Src p60, which is highly phosphorylated in the inactive form of the enzyme, while phosphorylated to a much lesser extent when the enzyme is active. For instance, a mutant of c-Src, in which Y527 is replaced by phenylalanine, is transforming and displays 5 to 10-fold elevated kinase activity compared to its normal counterpart. Csk has been identified as a Src related tyrosine kinase having both SH2 and SH3 domains and a catalytic domain but lacking sequences amino terminal to the SH3 domain as well as the carboxy terminal regulatory sequences. Csk phosphorylates Src on Y527 and also down regulates Fyn, Fyn and Lck by tyrosine phosphorylation of carboxy terminal regulatory sites. A Csk-like protein-tyrosine kinase of mouse cell origin (Ctk), also designated Ntk, and its human homolog, Lsk, have also been described. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Ctk of mouse and rat origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Ctk of mouse origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Ctk of mouse origin |
| Entrez Gene | 17179 |
|---|
Application
Western blot analysis of Ctk expression in BC3H1 (A) and EOC 20 (B) whole cell lysates.| Application Notes | recommended for detection of Ctk of mouse and rat origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
