Application
Western blot analysis of Fibrillarin expression in Hep G2 (A) and C4 (B) whole cell lysates, MOLT-4 (C) and K-562 (D) nuclear extracts and rat liver tissue extract (E).Application
Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear localization.| Product Name | Fibrillarin Antibody (H-140) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Fibrillarin is a widely occurring, basic, nonhistone protein that is localized exclusively in the fibrillar region of the nucleolus, including both the dense fibrillar and the fibrillar center regions. Fibrillarin is also expressed in HeLa cells, 3T3 cells, and human peripheral blood lymphocytes. In metaphase and anaphase, fibrillarin is found on putative chromosomal nucleolar regions (NORs). During telophase, fibrillarin is an early marker for the site of the newly forming nucleolus. The structure of fibrillarin includes an RNA-binding domain and an RNP consensus sequence, which is consistent with the association of fibrillarin with the U3 small nucleolar RNA. Fibrillarin is involved in processing rRNA transcripts in the nucleolus. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Fibrillarin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 61-200 of Fibrillarin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 61-200 of Fibrillarin of human origin |
| Entrez Gene | 2091 |
|---|
Application
Western blot analysis of Fibrillarin expression in Hep G2 (A) and C4 (B) whole cell lysates, MOLT-4 (C) and K-562 (D) nuclear extracts and rat liver tissue extract (E).
Application
Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear localization.| Application Notes | recommended for detection of Fibrillarin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
