Application
Western blot analysis of FAST-1 expression in non-transfected: (A) and human FAST-1 transfected: (B) 293T whole cell lysates.| Product Name | FAST-1/2 Antibody (H-280) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Xenopus winged-helix factor, xFAST-1 (forkhead activin signal transducer-1) is a transcription factor that forms a complex with the receptor-regulated Smad protein, Smad2, and directly binds to activin response elements on DNA (1,2). The human homolog FAST-1 and the corresponding mouse homolog, designated FAST-2, share significant sequence homology with xFAST-1, including a conserved N-terminal forkhead domain that consists of 110 amino acid residues and is essential for binding DNA and regulating transcription in embryogenesis, in tumorigenesis and in the maintenance of differentiated cell states (3,4). FAST-1 and FAST-2 also contain a distinct C-terminal Smad interaction domain that is required for the association with various Smad proteins, including Smad2, Smad3 and Smad4 (3,5). Expression of FAST-1 and FAST-2 is predominantly observed during early development, with lower levels detected in adult tissues (6,7). FAST-1 and FAST-2 mediated DNA binding is attenuated by both TFGß and activin, indicating that these FAST proteins mediate TFGß induced signal transduction (3). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of FAST-1 and FAST-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 86-365 of FAST-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 86-365 of FAST-1 of human origin |
| Entrez Gene | 8928 |
|---|
Application
Western blot analysis of FAST-1 expression in non-transfected: (A) and human FAST-1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of FAST-1 and FAST-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
