Application
Immunofluorescence staining of normal mouse heart frozen section showing blood vessel staining.Application
Western blot analysis of VWF expression in human platelet whole cell lysate.Application
Western blot analysis of purified human VWF.WB
(A, B) Representative image of CD31 (A) and vWF (B) staining (red) in the lesion epicenter and colocalization with the transplanted GFP-MSCs (green).| Product Name | VWF Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Von Willebrand disease is a congenital bleeding disorder caused by defects in the von Willebrand factor protein (VWF). VWF is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets, and it is involved in the coagulation of blood at injury sites. VWF acts as a carrier protein for Factor VIII, a cofactor required for coagulation, and it promotes platelet adhesion and aggregation. Several factors are known to stimulate the binding of VWF to platelets, including glycoprotein 1b, ristocetin, botrocetin, collagen, sulphatides and heparin. Of the several domains contained within VWF, the A1, A2 and A3 domains have been shown to mediate this activation. VWF is thought to undergo a variety of posttranslational modifications that influence the affinity and availability for Factor VII, including cleavage of the propeptide and formation of N-terminal intersubunit disulfide bonds. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| recommended for detection of VWF of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine | |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 2514-2813 mapping at the C-terminus of Von Willebrand factor (VWF) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 2514-2813 mapping at the C-terminus of Von Willebrand factor (VWF) of human origin |
| Entrez Gene | 7450 |
|---|
Application
Immunofluorescence staining of normal mouse heart frozen section showing blood vessel staining.
Application
Western blot analysis of VWF expression in human platelet whole cell lysate.
Application
Western blot analysis of purified human VWF.
WB
(A, B) Representative image of CD31 (A) and vWF (B) staining (red) in the lesion epicenter and colocalization with the transplanted GFP-MSCs (green).| Application Notes | recommended for detection of VWF of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
