Application
Western blot analysis of CD6 expression in non-transfected: (A) and human CD6 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CD6 expression in non-transfected: (A) and mouse CD6 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CD6 expression in human PBL whole cell lysate.Application
Western blot analysis of CD6 expression in CCRF-CEM (A), Jurkat (B) and MOLT-4 (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed Jurkat cells showing membrane localization.| Product Name | CD6 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | CD6 is a type I transmembrane glycoprotein that is present on mature thymocytes, peripheral T cells and a subset of B cells. The CD6 glycoprotein is tyrosine phosphorylated during TCR-mediated T-cell activation and the size difference between the CD6 forms is due in part to differences in phosphorylation state. CD6 protein contains a 24-amino acid signal sequence, three extracellular “scavenger receptor cysteine-rich” (SRCR) domains, a membrane-spanning domain, and a 44-amino acid cytoplasmic domain. CD6 shows significant homology to CD5. CD6, which is also found in brain and B cell chronic lymphocytic leukemias, plays an important role in interactions of thymocytes with thymic epithelial cells. CD6 molecules can physically associate with the TCR/CD3 complex. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB,IP,IF,ELISA |
| recommended for detection of CD6 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 369-668 of CD6 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 369-668 of CD6 of human origin |
| Entrez Gene | 923 |
|---|
Application
Western blot analysis of CD6 expression in non-transfected: (A) and human CD6 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CD6 expression in non-transfected: (A) and mouse CD6 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CD6 expression in human PBL whole cell lysate.
Application
Western blot analysis of CD6 expression in CCRF-CEM (A), Jurkat (B) and MOLT-4 (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed Jurkat cells showing membrane localization.| Application Notes | recommended for detection of CD6 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
