Application
Western blot analysis of CD13 expression in non-transfected 293T: (A), human CD13 transfected 293T: (B) and CCD-1064Sk (C) whole cell lysates.Application
Western blot analysis of CD13 expression in CCD-1064Sk whole cell lysate.| Product Name | CD13 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | CD13, or aminopeptidase N, is a type II transmembrane glycoprotein that is expressed on most cells of Myeloid origin, including monocytes, basophils, eosinophils, neutrophils and Myeloid leukemias. CD13 is also found on certain epithelial cells, fibroblasts and osteoclasts. CD13 acts as a zinc-binding metalloprotease that plays a role in digestion and may function in the inactivation of some regulatory peptides such as enkephalins. CD13 may play a role in the invasion of cancer cells by enhancing their invasive capacity and metastatic behavior. The activity of CD13 can be inactivated using specific inhibitors that evoke apoptosis of CD13-positive cancer cells. Basic fibroblast growth factor (bFGF) expression upregulates CD13 expression in human melanoma cells by activating both the Myeloid and the epithelial CD13 promoter. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CD13 of mouse, rat and human origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 668-967 mapping at the C-terminus of CD13 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 668-967 mapping at the C-terminus of CD13 of human origin |
| Entrez Gene | 290 |
|---|
Application
Western blot analysis of CD13 expression in non-transfected 293T: (A), human CD13 transfected 293T: (B) and CCD-1064Sk (C) whole cell lysates.
Application
Western blot analysis of CD13 expression in CCD-1064Sk whole cell lysate.| Application Notes | recommended for detection of CD13 of mouse, rat and human origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
