Application
Western blot analysis of XPA expression in GM637 whole cell lysate (A) and BJAB (B), MCF7 (C) and TF-1 (D) nuclear extracts.Application
Western blot analysis of whole cell lysates prepared from XPA expressing GM637 (A) and non-expressing XP12RO (B) cell lines. Cells kindly provided by J. Cleaver.Application
Western blot analysis of XPA expression in HeLa whole cell lysate.Application
Western blot analysis of XPA expression in BJAB nuclear extract.Application
Western blot analysis of XPA expression in non-transfected: (A) and mouse XPA transfected: (B) 293T whole cell lysates.Application
Western blot analysis of XPA expression in non-transfected control (A) and XPA siRNA transfected (B) HeLa cells. Blot probed with XPA (FL-273): . GAPDH (FL-335): used as specificity and loading control.| Product Name | XPA Antibody (FL-273) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Xeroderma pigmentosum (XP) is an autosomal recessive disorder characterized by a genetic predisposition to sunlight-induced skin cancer due to deficiencies in the DNA repair enzymes. The most frequent mutations are found in the XP genes of group A through G and group V, which encode nucleotide excision repair proteins. Nucleotide excision repair (NER) is the normal cellular response to DNA damage induced by UV irradiation and is disrupted in patients with XP. Xeroderma pigmentosum group A (XPA) is an essential NER factor that coordinates the collection of a preincision complex during the processing of DNA damage. XPA may also have a role in the repair of oxidized DNA bases. XPA is sensitive not only to the structure of the DNA double helix, but also to bulky groups incorporated into DNA. XPA forms a homodimer in the absence of DNA, but binds to DNA in both monomeric and dimeric forms. The dimerically bound XPA is much more efficient, so cells probably regulate XPA activity in a concentration-dependent manner. XPA deficient organisms cannot repair UV-induced DNA damage and thus acquire skin cancers by UV irradiation very easily. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB,IP,IF,IHC,ELISA |
| recommended for detection of XPA of mouse, rat, human and Xenopus origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-273 representing full length xeroderma pigmentosum group A protein of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-273 representing full length xeroderma pigmentosum group A protein of human origin |
| Entrez Gene | 7507 |
|---|
Application
Western blot analysis of XPA expression in GM637 whole cell lysate (A) and BJAB (B), MCF7 (C) and TF-1 (D) nuclear extracts.
Application
Western blot analysis of whole cell lysates prepared from XPA expressing GM637 (A) and non-expressing XP12RO (B) cell lines. Cells kindly provided by J. Cleaver.
Application
Western blot analysis of XPA expression in HeLa whole cell lysate.
Application
Western blot analysis of XPA expression in BJAB nuclear extract.
Application
Western blot analysis of XPA expression in non-transfected: (A) and mouse XPA transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of XPA expression in non-transfected control (A) and XPA siRNA transfected (B) HeLa cells. Blot probed with XPA (FL-273): . GAPDH (FL-335): used as specificity and loading control.| Application Notes | recommended for detection of XPA of mouse, rat, human and Xenopus origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
