Application
Western blot analysis of CAF-1 p150 expression in non-transfected: (A) and mouse CAF-1 p150 transfected: (B) 293T whole cell lysates and Jurkat nuclear extract (C).Application
Western blot analysis of CAF-1 p150 expression in HeLa (A) and K-562 (B) nuclear extracts.| Product Name | CAF-1 p150 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication. The p150 subunit of CAF-1 also supports the maintenance of heterochromatin, which requires the synthesis of both new histones and heterochromatin proteins and their orderly assembly during DNA replication. Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1å, HP1∫ and HP1©). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CAF-1 p150 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-200 mapping at the N-terminus of CAF-1 p150 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-200 mapping at the N-terminus of CAF-1 p150 of human origin |
| Entrez Gene | 10036 |
|---|
Application
Western blot analysis of CAF-1 p150 expression in non-transfected: (A) and mouse CAF-1 p150 transfected: (B) 293T whole cell lysates and Jurkat nuclear extract (C).
Application
Western blot analysis of CAF-1 p150 expression in HeLa (A) and K-562 (B) nuclear extracts.| Application Notes | recommended for detection of CAF-1 p150 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
