Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of TFIID expression in HeLa whole cell lysate.Application
Western blot analysis of TFIID expression in F9 whole cell lysate.Application
Western blot analysis of TFIID expression in non-transfected: (A) and mouse TFIID transfected: (B) 293T whole cell lysates.Application
Western blot analysis of TFIID expression in non-transfected: (A) and human TFIID transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human malignant glioma tissue showing nuclear staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
ChIP analysis of TFIID recruitment to genomic amplicons. Two (1,2) different human genomic TBP and three control amplicons (3-5) were analyzed by quantitative PCR (primer sequences available in on-line supplemental data). Data generated in collaboration with Drs. N. Trinklein and R. Myers, Stanford University (ENCODE Project).Application
Western blot analysis of TFIID p36 partially purified from human placental tissue.| Product Name | TFIID (TBP) Antibody (N-12) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the N-terminus of TFIID of human origin |
| Modification Notes | In eukaryotic systems, initiation of transcription from protein-coding genes is a complex process requiring RNA polymerase II and broad families of auxiliary transcription factors. Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, including TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH; and sequence-specific factors that regulate gene expression. The basal transcription factors and Pol II form a specific multiprotein complex near the transcription start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site. Binding of TFIID to the TATA element initiates assembly of the other factors into a pre-initiation complex. The TATA-binding subunit of TFIID (designated TFIIDt or TBP) from higher eukaryotes contains a highly conserved 180 amino acid C-terminal domain. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of TFIID (TBP) p36 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the N-terminus of TFIID of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the N-terminus of TFIID of human origin |
| Entrez Gene | 6908 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of TFIID expression in HeLa whole cell lysate.
Application
Western blot analysis of TFIID expression in F9 whole cell lysate.
Application
Western blot analysis of TFIID expression in non-transfected: (A) and mouse TFIID transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of TFIID expression in non-transfected: (A) and human TFIID transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human malignant glioma tissue showing nuclear staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
ChIP analysis of TFIID recruitment to genomic amplicons. Two (1,2) different human genomic TBP and three control amplicons (3-5) were analyzed by quantitative PCR (primer sequences available in on-line supplemental data). Data generated in collaboration with Drs. N. Trinklein and R. Myers, Stanford University (ENCODE Project).
Application
Western blot analysis of TFIID p36 partially purified from human placental tissue.| Application Notes | recommended for detection of TFIID (TBP) p36 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
