Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of TFIID expression in HeLa whole cell lysate.Application
Western blot analysis of TFIID expression in non-transfected: (A) and mouse TFIID transfected: (B) 293T whole cell lysates.Application
Western blot analysis of TFIID expression in non-transfected: (A) and human TFIID transfected: (B) 293T whole cell lysates.Application
Antibodies tested include Pol II (C-21): and TFIID (TBP) (SI-1): . CD3ε and Oct-2 promoter regions were employed as positive and negative controls, respectively. DNA was isolated from Rag-/- or WT thymocytes. Data kindly provided by J. Imbert and reproduced from Yeh, J.H., et al. 2002. Nucleic Acids Res. 30: 1944-1951, with permission from Oxford University Press.Application
Antibodies tested included NFκB p65 (A): , ATF-2 (N-96): , GCN5 (N-18): , Pol II (H-224): , TFIID (TBP)(SI-1): and CBP (A-22): . Data kindly provided by G. Mosialos.Application
Western blot analysis of TFIID (TBP) expression in mouse placenta tissue extract.| Product Name | TFIID (TBP) Antibody (SI-1) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against full length TFIID of human origin |
| Modification Notes | In eukaryotic systems, initiation of transcription from protein-coding genes is a complex process requiring RNA polymerase II and broad families of auxiliary transcription factors. Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, including TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH; and sequence-specific factors that regulate gene expression. The basal transcription factors and Pol II form a specific multiprotein complex near the transcription start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site. Binding of TFIID to the TATA element initiates assembly of the other factors into a pre-initiation complex. The TATA-binding subunit of TFIID (designated TFIIDt or TBP) from higher eukaryotes contains a highly conserved 180 amino acid C-terminal domain with all of the essential regions for DNA binding, transcription initiation and species specificity, and divergent N-terminal regions. The binding of TFIID to DNA is stimulated by direct interaction with TFIIA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of TFIID (TBP) p36 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against full length TFIID of human origin |
|---|---|
| Tissue Specificity | raised against full length TFIID of human origin |
| Entrez Gene | 6908 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of TFIID expression in HeLa whole cell lysate.
Application
Western blot analysis of TFIID expression in non-transfected: (A) and mouse TFIID transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of TFIID expression in non-transfected: (A) and human TFIID transfected: (B) 293T whole cell lysates.
Application
Antibodies tested include Pol II (C-21): and TFIID (TBP) (SI-1): . CD3ε and Oct-2 promoter regions were employed as positive and negative controls, respectively. DNA was isolated from Rag-/- or WT thymocytes. Data kindly provided by J. Imbert and reproduced from Yeh, J.H., et al. 2002. Nucleic Acids Res. 30: 1944-1951, with permission from Oxford University Press.
Application
Antibodies tested included NFκB p65 (A): , ATF-2 (N-96): , GCN5 (N-18): , Pol II (H-224): , TFIID (TBP)(SI-1): and CBP (A-22): . Data kindly provided by G. Mosialos.
Application
Western blot analysis of TFIID (TBP) expression in mouse placenta tissue extract.| Application Notes | recommended for detection of TFIID (TBP) p36 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
