Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing nuclear staining of cells in molecular layer and Purkinje cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of human recombinant Ski fusion protein.| Product Name | Ski Antibody (H-329) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 400-728 mapping at the C-terminus of Ski of human origin |
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | The Ski family of oncogenes includes Ski and Sno (Ski-related novel gene, or Ski-like). Three isoforms of human Sno (SnoN, SnoA and SnoI) and two isoforms in mouse (SnoN and SnoN2, also designated sno-dE3) are produced by alternative splicing of the SKIL gene. Ski family members are nuclear proteins that form homodimers and heterodimers, bind to DNA and function as transcriptional activators and repressors. These proteins consist of five tandem repeats in the C-terminal domain and two leucine zipper motifs that are responsible for efficient DNA binding, trimerization and cellular transformation. The Ski proteins regulate TGFß induced gene-specific transcriptional activation by effectively repressing Smad activity and, thereby, inhibit TGFß induced cell growth and extracellular matrix production. The amino terminus of Ski and SnoN preferentially associates with the MH2 domain of Smad2 and Smad4 of the Smad family of transcription factors, where they then recruit the transcriptional co-repressor protein N-CoR to the complex to inhibit transcription. Alternatively, Ski proteins are negatively regulated by various Smad proteins, as TGFß induces Smad3 accumulation in the nucleus, where it is then responsible for inducing the rapid degradation of SnoN and faciliating TGFß signaling pathways and Smad-activated gene transcription. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ChIPELISAGSIFIHCIPWB |
| WB:1:100~1:400 IP:1:20~1:100 IF:1:20~1:100 IHC:1:10~1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 400-728 mapping at the C-terminus of Ski of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 400-728 mapping at the C-terminus of Ski of human origin |
| Entrez Gene | 6497 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing nuclear staining of cells in molecular layer and Purkinje cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of human recombinant Ski fusion protein.| Application Notes | WB:1:100~1:400 IP:1:20~1:100 IF:1:20~1:100 IHC:1:10~1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
