Application
Western blot analysis of Miz-1 expression in HeLa whole cell lysate (A) and rat skeletal muscle tissue extract (B).| Product Name | Miz-1 Antibody (H-190) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The Myc family, including c-Myc-, N-Myc- and L-Myc, are nuclear proteins with relatively short half lives that contribute an important role in cellular processes such as proliferation, differentiation, apoptosis and transformation. The c-Myc protein activates transcription as part of a heteromeric complex with a number of interacting partners, including Max and Mxi 1; however the transforming properties of the Myc proto-oncogene are believed to be associated with Myc-mediated transcriptional repression. A POZ domain Zn finger protein, designated Miz-1 for Myc-interacting Zn finger protein-1, is a specific target of Myc-induced gene repression. Miz-1 interacts with Myc, but not Max or other Myc partners, and binding of Myc to Miz-1 requires the helix-loop-helix domain of Myc and a short amphipathic helix located in the carboxy-terminus of Miz-1. Miz-1 associates with DNA elements on the adenovirus major late and cyclin D1 promoters and activates transcription of both promoters. Expression of Miz-1 induces potent growth arrest function, and this latency is reversed by the addition of Myc. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of Miz-1 (Myc-interacting zinc finger protein 1) of mouse, rat and human origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 614-803 mapping at the C-terminus of Miz-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 614-803 mapping at the C-terminus of Miz-1 of human origin |
| Entrez Gene | 7709 |
|---|
Application
Western blot analysis of Miz-1 expression in HeLa whole cell lysate (A) and rat skeletal muscle tissue extract (B).| Application Notes | recommended for detection of Miz-1 (Myc-interacting zinc finger protein 1) of mouse, rat and human origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
