Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebral cortex tissue showing cytoplasmic staining of neuronal cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of PMR1 expression in HeLa (A), A-431 (B) and MDCK (C) whole cell lysates.| Product Name | PMR1 Antibody (H-200) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The Saccharomyces cerevisiae protein, PMR1, encodes P-type calcium transport ATPase, which localizes to the Golgi and regulates the intracellular transport of calcium and manganese. The human homologue, ATP2C1 (also designated SPLA in rat), also regulates the transport of calcium in the Golgi complex and is related to other P-type ATPases family members, such as the sarco(endo)plasmic calcium ATPase (SERCA) and the plasma membrane calcium ATPase (PCMA). PMR1 is a transmembrane protein that exists as two splice variants, which vary by 20 amino acids. PMR1 is mutated in Hailey-Hailey disease (HHD), which is an autosomal dominant disorder that is characterized by blisters and erosions of the skin. These findings provide further evidence that PMR1 plays a key role in maintaining the integrity of the epidermis by controlling intracellular calcium signaling. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of PMR1 of mouse, rat, human and canine origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 720-919 of PMR1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 720-919 of PMR1 of human origin |
| Entrez Gene | 27032 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebral cortex tissue showing cytoplasmic staining of neuronal cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of PMR1 expression in HeLa (A), A-431 (B) and MDCK (C) whole cell lysates.| Application Notes | recommended for detection of PMR1 of mouse, rat, human and canine origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
