Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bladder tissue showing nuclear and cytoplasmic staining of urothelial cells.Application
Western blot analysis of Rad17 expression in non-transfected: (A) and mouse Rad17 transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human breast tissue showing nuclear staining.Application
Western blot analysis of Rad17 expression in Jurkat (A), HeLa (B) and K-562 (C) nuclear extracts.| Product Name | Rad17 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | DNA damage results in the arrest of cell cycle progression, allowing the damaged DNA to be repaired prior to replication. Checkpoints exist at several cell cycle phase transitions to maintain this genetic integrity. Rad9, Rad17, Rad24 and Mec3 are involved in activating the G1 and G2 checkpoints. Pol2 (also known as Dun2), encoding the catalytic subunit of DNA polymerase epsilon, plays a role in activating the S phase checkpoint. The protein kinase Rad53 (also designated Spk1, Mec2 or Sad1) is essential for both G2 and S phase arrest. Activation of Rad53 is regulated by Mec1 (also known as Esr1 and Sad3), a homolog of the human ATM protein. Pds1 and Mad2 both regulate checkpoints associated with incomplete spindle replication. Dun1, another protein kinase, plays a role in transducing the DNA damage signal. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Rad17 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 371-670 mapping at the C-terminus of Rad17 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 371-670 mapping at the C-terminus of Rad17 of human origin |
| Entrez Gene | 5884 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bladder tissue showing nuclear and cytoplasmic staining of urothelial cells.
Application
Western blot analysis of Rad17 expression in non-transfected: (A) and mouse Rad17 transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human breast tissue showing nuclear staining.
Application
Western blot analysis of Rad17 expression in Jurkat (A), HeLa (B) and K-562 (C) nuclear extracts.| Application Notes | recommended for detection of Rad17 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
