Application
Western blot analysis of human recombinant GlyR α2 fusion protein.Application
Western blot analysis of GlyR α2 expression in F9 whole cell lysate.| Product Name | GlyR α2 Antibody (H-50) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | In the central nervous system (CNS), glycine-mediated inhibitory neurotransmission is essential to voluntary motor control and reflex responses. Glycine binds to glycine receptors (GlyR) in the post synaptic neuronal membranes. GlyR, ©-aminobutryic acid, serotonin and acetylcholine comprise an evolutionally conserved superfamily of ligand-gated ion channels. The pentameric subunit structure of GlyR consists of two types of glycosylated membrane proteins, å1 through å4 and ∫, and an associated peripheral membrane protein, which combine to form a chloride-selective ion channel. In humans, the composition of the pentamer changes from å2 subunits in the fetal CNS to å1 and ∫ subunits in the adult CNS. Fast potentiation of GlyR by intracellular Ca2+ in the brainstem and midbrain indicate an important role for Ca2+ in modulation glycinergic synapses. The genes encoding human GlyR å1, å2, å3 and ∫ subunits map to chromosomes 5q32, Xp22, 4q33 and 4q31, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of GlyR &alpha2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 371-420 mapping at the C-terminus of GlyR &alpha 2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 371-420 mapping at the C-terminus of GlyR α2 of human origin |
| Entrez Gene | 2742 |
|---|
Application
Western blot analysis of human recombinant GlyR α2 fusion protein.
Application
Western blot analysis of GlyR α2 expression in F9 whole cell lysate.| Application Notes | recommended for detection of GlyR &alpha2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
