Application
Western blot analysis of Elongin B expression in non-transfected 293T: (A), human Elongin B transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Immunofluorescence staining of normal mouse lymph node frozen section showing perinuclear staining.Application
Western blot analysis of Elongin B expression in HeLa (A) and Jurkat (B) whole cell lysates.| Product Name | Elongin B Antibody (FL-118) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Individuals harboring germline mutations in the tumor suppressor gene von Hippel-Lindau (VHL) exhibit an increased susceptibility to a variety of tumors including renal carcinoma, hemangio-blastoma of the central nervous system and pheochromocytoma. The Elongin (SIII) complex has been identified as the functional target of the VHL protein. Elongin (SIII) is a heterotrimer composed of a transcriptional active subunit designated Elongin A and two regulatory subunits designated Elongin B and Elongin C. VHL functions by binding to the Elongin B and C subunits, inhibiting the transcriptional efficacy of the Elongin (SIII) complex. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Elongin B of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-118 representing full length Elongin B of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-118 representing full length Elongin B of human origin |
| Entrez Gene | 6923 |
|---|
Application
Western blot analysis of Elongin B expression in non-transfected 293T: (A), human Elongin B transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Immunofluorescence staining of normal mouse lymph node frozen section showing perinuclear staining.
Application
Western blot analysis of Elongin B expression in HeLa (A) and Jurkat (B) whole cell lysates.| Application Notes | recommended for detection of Elongin B of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
