Application
Western blot analysis of Amphiphysin I expression in IMR-32 whole cell lysate.| Product Name | Amphiphysin I Antibody (H-230) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Amphiphysin is a brain-enriched protein that exhibits N-terminal lipid interaction and functions as a dimer. Amphiphysin contains a membrane bending BAR domain, a middle Clathrin and adaptor binding domain, and a C-terminal SH3 domain. In the brain, Amphiphysin I and II form heterodimers that bind to the Clathrin associated GTPase Dynamin via their SH3 domains. This association is essential for synaptic vesicle recycling in neurons, as it precedes the binding of Dynamin to the Clathrin-coated pits and the subsequent vesicle budding. In other tissues, Amphiphysin may play a key role in other membrane bending and curvature stabilization events. The mammalian Amphiphysins, Amphiphysin I and Amphiphysin II, have similar overall structure. A ubiquitous splice form of Amphiphysin II that does not contain Clathrin or adaptor interactions is highly expressed in muscle tissue and is involved in the formation and stabilization of the T tubule network. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Amphiphysin I of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 467-620 mapping near the C-terminus of Amphiphysin I of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 467-620 mapping near the C-terminus of Amphiphysin I of human origin |
| Entrez Gene | 273 |
|---|
Application
Western blot analysis of Amphiphysin I expression in IMR-32 whole cell lysate.| Application Notes | recommended for detection of Amphiphysin I of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
