Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach tissue showing cytoplasmic and nuclear staining of glandular cells.Application
Western blot analysis of BECN1 expression in non-transfected 293T: (A), mouse BECN1 transfected 293T: (B) and MCF7 (C) whole cell lysates.Application
Western blot analysis of BECN1 expression in non-transfected: (A) and human BECN1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of BECN1 expression in MCF7 whole cell lysate.Application
Western blot analysisof BECN1 expression in non-transfected control (A) and BECN1 siRNA transfected (B) HeLa cells. Blot probed with BECN1 (H-300): . β Tubulin (D-10): used as specificity and loading control.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse prostate tissue (A) and immunofluorescence staining of methanol-fixed NIH/3T3 cells (B) showing cytoplasmic localization.| Product Name | BECN1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | BECN1 (Beclin 1) is a coiled-coil protein that has been implicated as an inhibitor of tumorigenesis. BECN1, which associates with Bcl-2, plays a significant role in autophagy. Autophagy is the degradation of cellular proteins in the lysosomes, and when this pathway is suppressed, cell growth is deregulated. Autophagy is controlled by the same signal transduction pathway that induces the phosphorylation of the Ribosomal Protein S6, and both are mediated via amino acids. BECN1 expression in various carcinoma cell lines such as MCF7 is low, whereas it is ubiquitously expressed in normal breast tissue. In transfected MCF7 cells, BECN1 complements autophagocytosis and, subsequently, inhibits cellular proliferation. Additionally, BECN1 shares structural similarity to the yeast autophagy gene product, Apg6, and was one of the first mammalian proteins discovered to mediate autophagy. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of BECN1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of BECN1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of BECN1 of human origin |
| Entrez Gene | 8678 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach tissue showing cytoplasmic and nuclear staining of glandular cells.
Application
Western blot analysis of BECN1 expression in non-transfected 293T: (A), mouse BECN1 transfected 293T: (B) and MCF7 (C) whole cell lysates.
Application
Western blot analysis of BECN1 expression in non-transfected: (A) and human BECN1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of BECN1 expression in MCF7 whole cell lysate.
Application
Western blot analysisof BECN1 expression in non-transfected control (A) and BECN1 siRNA transfected (B) HeLa cells. Blot probed with BECN1 (H-300): . β Tubulin (D-10): used as specificity and loading control.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse prostate tissue (A) and immunofluorescence staining of methanol-fixed NIH/3T3 cells (B) showing cytoplasmic localization.| Application Notes | recommended for detection of BECN1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
