Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing cytoplasmic staining of hepatocytes.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of CYPOR expression in 293T (A) and Hela (B) whole cell lysates.Application
Western blot analysis of CYPOR expression in non-transfected 293T: (A), human CYPOR transfected 293T: (B) and A-431 (C) whole cell lysates.Application
Western blot analysis of CYPOR expression in rat liver (A) and mouse liver (B) tissue extracts.Application
Western blot analysis of CYPOR expression in MIA PaCa-2 whole cell lysate.| Product Name | CYPOR Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | P450 enzymes constitute a family of monooxygenase enzymes that are involved in the metabolism of a wide array of endogenous and xenobiotic compounds. Several P450 enzymes have been classified by sequence similarities as members of the CYP1A and CYP2A subfamilies. CYPOR, also known as cytochrome P450 reductase and NADPH cytochrome P450 reductase, is a microsomal enzyme responsible for the transfer of electrons from NADPH to cytochrome P450 enzymes during the P450 catalytic cycle. CYPOR is localized to the endoplasmic reticulum, where it is also able to transfer electrons to heme oxygenase and cytochrome b5. CYPOR is structurally related to two separate flavoprotein families, ferredoxin nucleotide reductase (FNR) and flavodoxin. Electron transfer of CYPOR requires the binding of two flavin cofactors, FAD and FMN, to the FNR and flavodoxin domains, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of CYPOR of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against amino acids 1-300 of CYPOR of human origin. |
|---|---|
| Tissue Specificity | raised against amino acids 1-300 of CYPOR of human origin. |
| Entrez Gene | 5447 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing cytoplasmic staining of hepatocytes.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of CYPOR expression in 293T (A) and Hela (B) whole cell lysates.
Application
Western blot analysis of CYPOR expression in non-transfected 293T: (A), human CYPOR transfected 293T: (B) and A-431 (C) whole cell lysates.
Application
Western blot analysis of CYPOR expression in rat liver (A) and mouse liver (B) tissue extracts.
Application
Western blot analysis of CYPOR expression in MIA PaCa-2 whole cell lysate.| Application Notes | recommended for detection of CYPOR of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
