Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of Calpastatin expression in non-transfected: (A) and mouse Calpastatin transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Calpastatin expression in non-transfected: (A) and human Calpastatin transfected: (B) 293 whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing cytoplasmic staining of surface epthelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of Calpastatin expression in non-transfected control (A) and Calpastatin siRNA transfected (B) HeLa cells. Blot probed with Calpastatin (H-300): . α-actinin (H-2): used as specificity and loading control.Application
Western blot analysis of Calpastatin expression in non-transfected: (A) and human Calpastatin transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Calpastatin expression in WEHI-231 whole cell lysate.| Product Name | Calpastatin Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Calpains are nonlysosomal, calcium-activated intracellular cysteine proteases that mediate specific Ca2+-dependent processes including cell fusion, mitosis and meiosis. Calpains are heterodimers of a small regulatory subunit and one of three large catalytic subunits, designated Calpain 1, Calpain 2 and Calpain p94. Calpain 1 is an intracellular calcium-dependent protease that cleaves cytoskeletal and submembranous proteins. Calpain-1 co-localizes with human leukocyte antigen-DR (HLA-DR) on activated microglia in the aging brain. Cal-pain influences the process of spermatogenesis and the events preceding fertilization, such as the acrosome reaction. Calpastatin regulates Calpain by inhibiting both the proteolytic activity of Calpain and its binding to membranes. Calpastatin exists in two types, tissue type and erythrocyte type, resulting from both alternative splicing and proteolytic processing. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Calpastatin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 409-708 mapping at the C-terminus of Calpastatin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 409-708 mapping at the C-terminus of Calpastatin of human origin |
| Entrez Gene | 831 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of Calpastatin expression in non-transfected: (A) and mouse Calpastatin transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Calpastatin expression in non-transfected: (A) and human Calpastatin transfected: (B) 293 whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing cytoplasmic staining of surface epthelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of Calpastatin expression in non-transfected control (A) and Calpastatin siRNA transfected (B) HeLa cells. Blot probed with Calpastatin (H-300): . α-actinin (H-2): used as specificity and loading control.
Application
Western blot analysis of Calpastatin expression in non-transfected: (A) and human Calpastatin transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Calpastatin expression in WEHI-231 whole cell lysate.| Application Notes | recommended for detection of Calpastatin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
