Application
Western blot analysis of Renin expression in KNRK whole cell lysate (A) and mouse liver (B) and rat kidney (C) tissue extracts.Application
Western blot analysis of Renin expression in non-transfected 293T: (A), human Renin transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of Renin expression in rat kidney tissue extract.| Product Name | Renin Antibody (H-105) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 116-220 mapping to an internal region of Renin of human origin |
| Modification Notes | Renin is a highly specific endopeptidase that mediates the cleavage of the circulating substrate angiotensinogen to yield Angiotensin I. Angiotensin-converting enzyme I (ACE) then completes the conversion from Angiotensin I to Angiotensin II which is significant in the regulation of electrolyte balance and blood pressure. Sympathetic stimulation (∫1-adrenergic receptors), renal artery hypotension and decreases in sodium delivery to the distal tubules of the kidney signal the release of Renin. The Renin-Angiotensin system (RAS) is essential for regulating blood volume, arterial pressure and normal cardiac and vascular function. Renin is synthesized and secreted by modified smooth muscle cells in the juxtaglomerular apparatus (JGA) of the kidney. Expression of Renin in other tissues, including brain, has been verified although the homeostatic role it may play is yet to be firmly established. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Renin of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 116-220 mapping to an internal region of Renin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 116-220 mapping to an internal region of Renin of human origin |
| Entrez Gene | 5972 |
|---|
Application
Western blot analysis of Renin expression in KNRK whole cell lysate (A) and mouse liver (B) and rat kidney (C) tissue extracts.
Application
Western blot analysis of Renin expression in non-transfected 293T: (A), human Renin transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of Renin expression in rat kidney tissue extract.| Application Notes | recommended for detection of Renin of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
