Application
Western blot analysis of c-Fms/CSF-1R expression in non-transfected: (A) and mouse c-Fms/CSF-1R transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of methanol-fixed RAW 264.7 cells showing membrane staining.Application
Western blot analysis of CSF-1R expression in RAW 264.7 whole cell lysate. The two major bands represent differentially glycosylated receptors.| Product Name | c-Fms/CSF-1R Antibody (C-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | c-Fms/CSF-1R, also designated macrophage colony-stimulating factor receptor (M-CSFR), FIM2 or CD115, is a transmembrane tyrosine kinase receptor belonging to the CSF1/PDGF receptor family. It is encoded by the c-Fms proto-oncogene and is expressed in mononuclear phagocytes, oocytes, decidual cells, trophoblastic cells and some myoblasts. It is important for growth and differentiation of myeloid cells and its function can be regulated by SLAP-2. c-Fms/CSF-1R is responsible for mediating all of the functions of M-CSF. M-CSF is a glycoprotein required for the proliferation and differentiation of mononuclear phagocytes, including osteoclasts. M-CSF has also been identified as an important mediator of the inflammatory response and can regulate the release of proinflammatory cytokines from macrophages. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of c-Fms/CSF-1R of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, feline and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of c-Fms/CSF-1R of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of c-Fms/CSF-1R of human origin |
| Entrez Gene | 1436 |
|---|
Application
Western blot analysis of c-Fms/CSF-1R expression in non-transfected: (A) and mouse c-Fms/CSF-1R transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed RAW 264.7 cells showing membrane staining.
Application
Western blot analysis of CSF-1R expression in RAW 264.7 whole cell lysate. The two major bands represent differentially glycosylated receptors.| Application Notes | recommended for detection of c-Fms/CSF-1R of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, feline and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
