Application
Western blot analysis of PKC expression in HeLa whole cell lysate.Application
Western blot analysis of PKC expression in 293T (A) and HeLa (B) whole cell lysates.Application
Immunofluorescence staining of normal mouse liver frozen section showing membrane and cytoplasmic staining.Application
Western blot analysis of PKC expression in 3611-RF (A) and NIH/3T3 (B) whole cell lysates.| Product Name | PKC Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into many different isoforms (å, ∫I, ∫II, ©, ∂, é, Ω, h, †, λ/î, µ and n). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC ∂ and é are independent of Ca++. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of all PKC family members of mouse, rat, human, Drosophila and Xenopus origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 373-672 mapping at the C-terminus of PKC &alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 373-672 mapping at the C-terminus of PKC α of human origin |
| Entrez Gene | 5578 |
|---|
Application
Western blot analysis of PKC expression in HeLa whole cell lysate.
Application
Western blot analysis of PKC expression in 293T (A) and HeLa (B) whole cell lysates.
Application
Immunofluorescence staining of normal mouse liver frozen section showing membrane and cytoplasmic staining.
Application
Western blot analysis of PKC expression in 3611-RF (A) and NIH/3T3 (B) whole cell lysates.| Application Notes | recommended for detection of all PKC family members of mouse, rat, human, Drosophila and Xenopus origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
