Application
Western blot analysis of IKK-i expression in non-transfected: (A) and mouse IKK-i transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of normal mouse liver frozen section showing cytoplasmic staining.Application
Western blot analysis of IKK-i expression in RAW 309 Cr.1 whole cell lysate.| Product Name | IKK-i Antibody (H-116) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The transcription factor NF˚B is retained in the cytoplasm in an inactive form by the inhibitory protein I˚B. Activation of NF˚B requires that I˚B be phosphorylated on specific serine residues, which results in targeted degradation of I˚B. I˚B kinase å (IKKå), previously designated CHUK, interacts with I˚B-å and specifically phosphorylates I˚B-å on the sites that trigger its degradation, Serines 32 and 36. The functional IKK complex contains three subunits, IKKå, IKK∫ and IKK© (also designated NEMO), and each appear to make essential contributions to I˚B phosphorylation. IKK-i is a serine/threonine kinase that shares homology with IKKå and IKK∫. IKK-i is primarily expressed in immune cells and is induced by lipopolysaccharide and by proinflammatory cytokines including TNFå, IL-1 and IL-6. Overexpression of IKK-i has been shown to result in phosphorylation of I˚Bå on Ser 32 and Ser 36, and in NF˚B activation, suggesting that IKK-i may act as an I˚B kinase in the immune system. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of IKK-i of mouse, rat and human origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 440-555 mapping near the C-terminus of IKK-i of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 440-555 mapping near the C-terminus of IKK-i of human origin |
| Entrez Gene | 9641 |
|---|
Application
Western blot analysis of IKK-i expression in non-transfected: (A) and mouse IKK-i transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of normal mouse liver frozen section showing cytoplasmic staining.
Application
Western blot analysis of IKK-i expression in RAW 309 Cr.1 whole cell lysate.| Application Notes | recommended for detection of IKK-i of mouse, rat and human origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
