Application
Western blot analysis of Syndecan-4 expression in non-transfected 293: (A), human Syndecan-4 transfected 293: (B) and A-673 (C) whole cell lysates.Application
Western blot analysis of Syndecan-4 expression in non-transfected 293T: (A), mouse Syndecan-4 transfected 293T: (B) and Hep G2 (C) whole cell lysates.Application
Western blot analysis of Syndecan-4 expression in non-transfected control (A) and Syndecan-4 siRNA transfected (B) HeLa cells. Blot probed with Syndecan-4 (H-140): . GAPDH (FL-335): used as specificity and loading control.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse liver tissue showing membrane localization.Application
Western blot analysis of Syndecan-4 expression in A-673 whole cell lysate.| Product Name | Syndecan-4 Antibody (H-140) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Syndecans are type I integral membrane proteoglycans that contain both chondroitin sulfate and heparan sulfate groups. Syndecans are involved in cell-extracellular matrix adhesion and growth factor binding. Syndecan-1 (SYND1, also called CD138) is an extracellular matrix receptor which binds to collagens, Fibronectin and Thrombospondin. Syndecan-1 and Syndecan-3 (also designated N-Syndecan) interact with MK (midkine), a growth/differentiation factor invloved in embryogenesis of the central nervous system. Syndecan-2 (also designated fibroglycan) is highly expressed at areas of high morphogenetic activity, such as epithelial-mesenchymal interfaces and the prechondrogenic and preosteogenic mesenchymal condensations. Syndecan-4 (also designated amphiglycan or ryudocan) functions cooperativley with integrins in the processes of cell spreading, focal adhesion assembly and Actin stress fiber assembly. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Syndecan-4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-140 mapping at the N-terminus of Syndecan-4 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-140 mapping at the N-terminus of Syndecan-4 of human origin |
| Entrez Gene | 6385 |
|---|
Application
Western blot analysis of Syndecan-4 expression in non-transfected 293: (A), human Syndecan-4 transfected 293: (B) and A-673 (C) whole cell lysates.
Application
Western blot analysis of Syndecan-4 expression in non-transfected 293T: (A), mouse Syndecan-4 transfected 293T: (B) and Hep G2 (C) whole cell lysates.
Application
Western blot analysis of Syndecan-4 expression in non-transfected control (A) and Syndecan-4 siRNA transfected (B) HeLa cells. Blot probed with Syndecan-4 (H-140): . GAPDH (FL-335): used as specificity and loading control.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse liver tissue showing membrane localization.
Application
Western blot analysis of Syndecan-4 expression in A-673 whole cell lysate.| Application Notes | recommended for detection of Syndecan-4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
