Application
Western blot analysis of TLR3 expression in non-transfected control (A) and TLR3 siRNA transfected (B) HeLa cells. Blot probed with TLR3 (H-125): . α-actinin (H-2): used as specificity and loading control.| Product Name | TLR3 Antibody (H-125) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Six human homologs of the Drosophila Toll receptor were initially identified based on their sequence similarities and designated Toll-like receptors (TLR). Toll receptors are involved in mediating dorsoventral polarization in the developing Drosophila embryo and also participate in the host immunity. The TLR family of proteins are characterized by a highly conserved Toll homology (TH) domain, which is essential for Toll-induced signal transduction. Expression of TLR receptors is highest in peripheral blood leukocytes, macrophages and monocytes. TLR1, as well as the other TLR family members, are type I transmembrane receptors that characteristically contain an extracellular domain consisting of several leucine-rich regions along with a single cytoplasmic Toll/IL-1R-like domain. TLR2 and TLR4 are activated in response to lipopolysacchride (LPS) stimulation, which results in the activation and translocation of NF˚B and suggests that these receptors are involved in mediating inflammatory responses. TLR3 is highly expressed in placenta and pancreas, and is limited to the dendritic subpopulation of leukocytes. TLR3 recognizes dsRNA associated with viral infection and induces activation of NF˚B and production of type I interferons, which suggests that it may play a role in host defense against viruses. TLR6 is highly homologous to TLR1, sharing greater than 65% sequence identity. Like other members of TLR family, TLR6 induces NF˚B signaling upon activation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB,IP,IF,ELISA |
| recommended for detection of TLR3 of human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 653-777 mapping near the C-terminus of TLR3 (Toll-like receptor 3) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 653-777 mapping near the C-terminus of TLR3 (Toll-like receptor 3) of human origin |
| Entrez Gene | 7098 |
|---|
Application
Western blot analysis of TLR3 expression in non-transfected control (A) and TLR3 siRNA transfected (B) HeLa cells. Blot probed with TLR3 (H-125): . α-actinin (H-2): used as specificity and loading control.| Application Notes | recommended for detection of TLR3 of human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
