Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human appendix tissue showing cytoplasmic staining of glandular and lymphoid cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of normal mouse intestine frozen section showing membrane staining.| Product Name | cGKII Antibody (H-120) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | cGKII (cGMP-dependent protein kinase type II) is a major receptor of intracellular cGMP and mediates a plethora of physiological responses (1-3). cGKII contains a conserved leucine zipper motif at the amino-terminus (4,5). It is expressed in small intestine, colon, prostate, and human brain tissues, and the cGKII gene maps to chromosome 4q13.1-q21.1 (3). cGKII has been shown to regulate the ion transport system in the intestine (6,7). Myristoylation of the penultimate glycine in cGKII appears to be essential for directing cGKII to the membrane, since cGKII is devoid of any hydrophobic transmembrane domains (7). The translocation of cGKII from the cytosol to the membrane allows it to function properly in regulating intestinal ion transport (7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of cGKII of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-120 of cGKII of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-120 of cGKII of human origin |
| Entrez Gene | 5593 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human appendix tissue showing cytoplasmic staining of glandular and lymphoid cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing membrane staining.| Application Notes | recommended for detection of cGKII of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
