Application
Western blot analysis of SPCS3 expression in non-transfected: (A) and human SPCS3 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of SPCS3 expression in non-transfected: (A) and mouse SPCS3 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of SPCS3 expression in non-transfected: (A) and human SPCS3 transfected: (B) 293T whole cell lysates.| Product Name | SPCS3 Antibody (E-14) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | SPCS3 (signal peptidase complex subunit 3), also known as SPC3, is a 180 amino acid single-pass type II membrane protein that localizes to both the microsome and the endoplasmic reticulum (ER) and belongs to the SPCS (signal peptidase complex subunit) family. Existing as a component of the microsomal signal peptidase complex which consists of five members, SPCS3 functions to remove signal peptides from proteins that are translated to the lumen of the ER. The gene encoding SPCS3 maps to human chromosome 4, which encodes nearly 6% of the human genome and has the largest gene deserts (regions of the genome with no protein encoding genes) of all of the human chromosomes. Defects in some of the genes located on chromosome 4 are associated with Huntington's disease, Ellis-van Creveld syndrome, methylmalonic acidemia and polycystic kidney disease. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of SPCS3 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with SPCS2 also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of SPCS3 of human origin |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of SPCS3 of human origin |
| Entrez Gene | 60559 |
|---|
Application
Western blot analysis of SPCS3 expression in non-transfected: (A) and human SPCS3 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of SPCS3 expression in non-transfected: (A) and mouse SPCS3 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of SPCS3 expression in non-transfected: (A) and human SPCS3 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of SPCS3 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with SPCS2 also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
