Application
Immunofluorescence staining of methanol-fixed KNRK cells showing nuclear localization.Application
Western blot analysis of HDAC5 expression in Jurkat nuclear extract.| Product Name | HDAC5 Antibody (H-74) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBPassociated factor), p300/CBP, HAT1, and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3-6, have been identified as histone deacetylases. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of HDAC5 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 371-443 mapping near the N-terminus of HDAC5 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 371-443 mapping near the N-terminus of HDAC5 of human origin |
| Entrez Gene | 10014 |
|---|
Application
Immunofluorescence staining of methanol-fixed KNRK cells showing nuclear localization.
Application
Western blot analysis of HDAC5 expression in Jurkat nuclear extract.| Application Notes | recommended for detection of HDAC5 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
