Application
Western blot analysis of Topo IIIβ-1 expression in mouse testis tissue extract.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing nuclear and cytoplasmic staining of cells in glomeruli and tubuli at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of Topo IIIβ-1 expression in IMR-32 nuclear extract.| Product Name | Topo IIIβ-1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA by transiently breaking and rejoining DNA strands. Although DNA topoisomerase I (Topo I) and Topo II relax both positive and negative supercoils, Topo III relaxes only negative supercoils. Topo IIIå exists as a long and a short isoform, which are produced by alternative splicing. Topo IIIå, which localizes to the nucleolus, is constitutively expressed and remains at high levels throughout the cell cycle in HL-60 cells. Topo III∫ exists as three isoforms, ∫-1, ∫-2 and ∫-3, also produced by alternative splicing. Topo III∫-1 is expressed in testis, heart, and skeletal muscle, whereas Topo III∫-2 is expressed in thymus, kidney and pancreas. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of DNA Topoisomerase III&beta-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 563-862 of Topo III&beta -1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 563-862 of Topo IIIβ-1 of human origin |
| Entrez Gene | 8940 |
|---|
Application
Western blot analysis of Topo IIIβ-1 expression in mouse testis tissue extract.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing nuclear and cytoplasmic staining of cells in glomeruli and tubuli at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of Topo IIIβ-1 expression in IMR-32 nuclear extract.| Application Notes | recommended for detection of DNA Topoisomerase III&beta-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
