Application
Western blot analysis of Mat1 expression in non-transfected 293T: (A), human Mat1 transfected 293T: (B) and A-431 (C) whole cell lysates.Application
Western blot analysis of Mat1 expression in A-431 (A) and NIH/3T3 (B) whole cell lysates and His-tagged human recombinant Mat1 (C).| Product Name | Mat1 Antibody (C-167) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Progression through the cell cycle requires activation of a series of enzymes designated cyclin dependent kinases (Cdks). The monomeric catalytic subunit, Cdk2, a critical enzyme for initiation of cell cycle progression, is completely inactive. Partial activation is achieved by the binding of regulatory cyclins such as cyclin D1, while full activation requires phosphorylation at Thr 160. The enzyme responsible for phosphorylation of Thr 160 in Cdk2 and also Thr 161 in Cdc2 p34, designated Cdk-activating kinase (CAK), has been partially purified and shown to be comprised of a catalytic subunit, a regulatory subunit and a subunit of unknown function. The regulatory subunit is a novel cyclin (cyclin H) and is required for activation of Cdk7. This previously undescribed protein, now termed Mat1, has been cloned as a protein that associates with the cyclin H-Cdk7 nuclear complex at all stages of the cell cycle. Cyclin H-Cdk7-Mat1 complexes display kinase activity towards Cdk activation domains, and the carboxy terminus of RNA polymerase II. Mat1 appears to constitute the first example of an assembly factor, essential for the formation of an active Cdk-cyclin complex. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Mat1 p36 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 167-309 of Mat1 p36 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 167-309 of Mat1 p36 of human origin |
| Entrez Gene | 4331 |
|---|
Application
Western blot analysis of Mat1 expression in non-transfected 293T: (A), human Mat1 transfected 293T: (B) and A-431 (C) whole cell lysates.
Application
Western blot analysis of Mat1 expression in A-431 (A) and NIH/3T3 (B) whole cell lysates and His-tagged human recombinant Mat1 (C).| Application Notes | recommended for detection of Mat1 p36 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
